<!DOCTYPE html><html lang="zh-CN" data-theme="light"><head><meta charset="UTF-8"><meta http-equiv="X-UA-Compatible" content="IE=edge"><meta name="viewport" content="width=device-width, initial-scale=1.0,viewport-fit=cover"><title>第一篇博客 | 莫为轻阴便拟归</title><meta name="author" content="Mingyanlee"><meta name="copyright" content="Mingyanlee"><meta name="format-detection" content="telephone=no"><meta name="theme-color" content="#ffffff"><meta name="description" content="#建立博客的原因 想记录自己的点滴进步，也是一个时光轴。无论如何，不断进步，这个博客的存在也是提醒，是督促。 建立博客之后怎么做在博客中准备分成学习和生活感想两个版块。学习方面记录每天读的文献，学习的编程，做的实验技术；生活方面记录一下每天的平淡点滴，是时光的见证。 文献学习### Hepatic cytochrome P450 8B1 and cholic acid potentiate int">
<meta property="og:type" content="article">
<meta property="og:title" content="第一篇博客">
<meta property="og:url" content="http://example.com/2024/01/31/%E7%AC%AC%E4%B8%80%E7%AF%87%E5%8D%9A%E5%AE%A2/index.html">
<meta property="og:site_name" content="莫为轻阴便拟归">
<meta property="og:description" content="#建立博客的原因 想记录自己的点滴进步，也是一个时光轴。无论如何，不断进步，这个博客的存在也是提醒，是督促。 建立博客之后怎么做在博客中准备分成学习和生活感想两个版块。学习方面记录每天读的文献，学习的编程，做的实验技术；生活方面记录一下每天的平淡点滴，是时光的见证。 文献学习### Hepatic cytochrome P450 8B1 and cholic acid potentiate int">
<meta property="og:locale" content="zh_CN">
<meta property="og:image" content="https://i.loli.net/2021/02/24/5O1day2nriDzjSu.png">
<meta property="article:published_time" content="2024-01-31T06:42:33.000Z">
<meta property="article:modified_time" content="2024-02-13T16:22:38.655Z">
<meta property="article:author" content="Mingyanlee">
<meta name="twitter:card" content="summary">
<meta name="twitter:image" content="https://i.loli.net/2021/02/24/5O1day2nriDzjSu.png"><link rel="shortcut icon" href="/img/favicon.png"><link rel="canonical" href="http://example.com/2024/01/31/%E7%AC%AC%E4%B8%80%E7%AF%87%E5%8D%9A%E5%AE%A2/index.html"><link rel="preconnect" href="//cdn.jsdelivr.net"/><link rel="preconnect" href="//busuanzi.ibruce.info"/><link rel="stylesheet" href="/css/index.css?v=4.12.0"><link rel="stylesheet" href="https://cdn.jsdelivr.net/npm/@fortawesome/fontawesome-free@6.5.1/css/all.min.css"><link rel="stylesheet" href="https://cdn.jsdelivr.net/npm/@fancyapps/ui@5.0.32/dist/fancybox/fancybox.min.css" media="print" onload="this.media='all'"><script>const GLOBAL_CONFIG = {
  root: '/',
  algolia: undefined,
  localSearch: undefined,
  translate: {"defaultEncoding":2,"translateDelay":0,"msgToTraditionalChinese":"繁","msgToSimplifiedChinese":"簡"},
  noticeOutdate: undefined,
  highlight: {"plugin":"highlight.js","highlightCopy":true,"highlightLang":true,"highlightHeightLimit":false},
  copy: {
    success: '复制成功',
    error: '复制错误',
    noSupport: '浏览器不支持'
  },
  relativeDate: {
    homepage: false,
    post: false
  },
  runtime: '',
  dateSuffix: {
    just: '刚刚',
    min: '分钟前',
    hour: '小时前',
    day: '天前',
    month: '个月前'
  },
  copyright: undefined,
  lightbox: 'fancybox',
  Snackbar: undefined,
  infinitegrid: {
    js: 'https://cdn.jsdelivr.net/npm/@egjs/infinitegrid@4.11.0/dist/infinitegrid.min.js',
    buttonText: '加载更多'
  },
  isPhotoFigcaption: false,
  islazyload: false,
  isAnchor: false,
  percent: {
    toc: true,
    rightside: false,
  },
  autoDarkmode: false
}</script><script id="config-diff">var GLOBAL_CONFIG_SITE = {
  title: '第一篇博客',
  isPost: true,
  isHome: false,
  isHighlightShrink: false,
  isToc: true,
  postUpdate: '2024-02-14 00:22:38'
}</script><script>(win=>{
      win.saveToLocal = {
        set: (key, value, ttl) => {
          if (ttl === 0) return
          const now = Date.now()
          const expiry = now + ttl * 86400000
          const item = {
            value,
            expiry
          }
          localStorage.setItem(key, JSON.stringify(item))
        },
      
        get: key => {
          const itemStr = localStorage.getItem(key)
      
          if (!itemStr) {
            return undefined
          }
          const item = JSON.parse(itemStr)
          const now = Date.now()
      
          if (now > item.expiry) {
            localStorage.removeItem(key)
            return undefined
          }
          return item.value
        }
      }
    
      win.getScript = (url, attr = {}) => new Promise((resolve, reject) => {
        const script = document.createElement('script')
        script.src = url
        script.async = true
        script.onerror = reject
        script.onload = script.onreadystatechange = function() {
          const loadState = this.readyState
          if (loadState && loadState !== 'loaded' && loadState !== 'complete') return
          script.onload = script.onreadystatechange = null
          resolve()
        }

        Object.keys(attr).forEach(key => {
          script.setAttribute(key, attr[key])
        })

        document.head.appendChild(script)
      })
    
      win.getCSS = (url, id = false) => new Promise((resolve, reject) => {
        const link = document.createElement('link')
        link.rel = 'stylesheet'
        link.href = url
        if (id) link.id = id
        link.onerror = reject
        link.onload = link.onreadystatechange = function() {
          const loadState = this.readyState
          if (loadState && loadState !== 'loaded' && loadState !== 'complete') return
          link.onload = link.onreadystatechange = null
          resolve()
        }
        document.head.appendChild(link)
      })
    
      win.activateDarkMode = () => {
        document.documentElement.setAttribute('data-theme', 'dark')
        if (document.querySelector('meta[name="theme-color"]') !== null) {
          document.querySelector('meta[name="theme-color"]').setAttribute('content', '#0d0d0d')
        }
      }
      win.activateLightMode = () => {
        document.documentElement.setAttribute('data-theme', 'light')
        if (document.querySelector('meta[name="theme-color"]') !== null) {
          document.querySelector('meta[name="theme-color"]').setAttribute('content', '#ffffff')
        }
      }
      const t = saveToLocal.get('theme')
    
        if (t === 'dark') activateDarkMode()
        else if (t === 'light') activateLightMode()
      
      const asideStatus = saveToLocal.get('aside-status')
      if (asideStatus !== undefined) {
        if (asideStatus === 'hide') {
          document.documentElement.classList.add('hide-aside')
        } else {
          document.documentElement.classList.remove('hide-aside')
        }
      }
    
      const detectApple = () => {
        if(/iPad|iPhone|iPod|Macintosh/.test(navigator.userAgent)){
          document.documentElement.classList.add('apple')
        }
      }
      detectApple()
    })(window)</script><meta name="generator" content="Hexo 6.3.0"></head><body><div id="sidebar"><div id="menu-mask"></div><div id="sidebar-menus"><div class="avatar-img is-center"><img src="https://i.loli.net/2021/02/24/5O1day2nriDzjSu.png" onerror="onerror=null;src='/img/friend_404.gif'" alt="avatar"/></div><div class="sidebar-site-data site-data is-center"><a href="/archives/"><div class="headline">文章</div><div class="length-num">2</div></a><a href="/tags/"><div class="headline">标签</div><div class="length-num">0</div></a><a href="/categories/"><div class="headline">分类</div><div class="length-num">0</div></a></div><hr class="custom-hr"/><div class="menus_items"><div class="menus_item"><a class="site-page" href="/"><i class="fa-fw fas fa-home"></i><span> 首页</span></a></div><div class="menus_item"><a class="site-page" href="/archives/"><i class="fa-fw fas fa-archive"></i><span> 时间轴</span></a></div><div class="menus_item"><a class="site-page" href="/tags/"><i class="fa-fw fas fa-tags"></i><span> 标签</span></a></div><div class="menus_item"><a class="site-page" href="/categories/"><i class="fa-fw fas fa-folder-open"></i><span> 分类</span></a></div></div></div></div><div class="post" id="body-wrap"><header class="post-bg" id="page-header"><nav id="nav"><span id="blog-info"><a href="/" title="莫为轻阴便拟归"><span class="site-name">莫为轻阴便拟归</span></a></span><div id="menus"><div class="menus_items"><div class="menus_item"><a class="site-page" href="/"><i class="fa-fw fas fa-home"></i><span> 首页</span></a></div><div class="menus_item"><a class="site-page" href="/archives/"><i class="fa-fw fas fa-archive"></i><span> 时间轴</span></a></div><div class="menus_item"><a class="site-page" href="/tags/"><i class="fa-fw fas fa-tags"></i><span> 标签</span></a></div><div class="menus_item"><a class="site-page" href="/categories/"><i class="fa-fw fas fa-folder-open"></i><span> 分类</span></a></div></div><div id="toggle-menu"><a class="site-page" href="javascript:void(0);"><i class="fas fa-bars fa-fw"></i></a></div></div></nav><div id="post-info"><h1 class="post-title">第一篇博客</h1><div id="post-meta"><div class="meta-firstline"><span class="post-meta-date"><i class="far fa-calendar-alt fa-fw post-meta-icon"></i><span class="post-meta-label">发表于</span><time class="post-meta-date-created" datetime="2024-01-31T06:42:33.000Z" title="发表于 2024-01-31 14:42:33">2024-01-31</time><span class="post-meta-separator">|</span><i class="fas fa-history fa-fw post-meta-icon"></i><span class="post-meta-label">更新于</span><time class="post-meta-date-updated" datetime="2024-02-13T16:22:38.655Z" title="更新于 2024-02-14 00:22:38">2024-02-14</time></span></div><div class="meta-secondline"><span class="post-meta-separator">|</span><span class="post-meta-pv-cv" id="" data-flag-title="第一篇博客"><i class="far fa-eye fa-fw post-meta-icon"></i><span class="post-meta-label">阅读量:</span><span id="busuanzi_value_page_pv"><i class="fa-solid fa-spinner fa-spin"></i></span></span></div></div></div></header><main class="layout" id="content-inner"><div id="post"><article class="post-content" id="article-container"><p>#<font face="楷体" color="green" size="6">建立博客的原因</font></p>
<p><font face="楷体" color="blue" size="3">想记录自己的点滴进步，也是一个时光轴。<br>无论如何，不断进步，这个博客的存在也是提醒，是督促。</font></p>
<h2 id="建立博客之后怎么做"><a href="#建立博客之后怎么做" class="headerlink" title="建立博客之后怎么做"></a><font face="楷体" color="green" size="6">建立博客之后怎么做</font></h2><p><font face="楷体" color="blue" size="3">在博客中准备分成学习和生活感想两个版块。学习方面记录每天读的文献，学习的编程，做的实验技术；生活方面记录一下每天的平淡点滴，是时光的见证。</font></p>
<h2 id="文献学习"><a href="#文献学习" class="headerlink" title="文献学习"></a>文献学习</h2><p>###<em><strong><font face="Times New Roman"> Hepatic cytochrome P450 8B1 and cholic acid potentiate intestinal epithelial injury in colitis by supressing intesitnal stem cell renewal </font></strong></em></p>
<hr>
<p><font face="楷体" color="black" size="4">结果1，2，3在思维导图里不易上传就略过，直接到结果4<br><font face="Times New Roman">Results4</font>  <font face="楷体" color="black" size="4">CA蓄积引发Lgr5 +肠道干细胞丢失，阻碍上皮再生</font></font></p>
<blockquote>
<p>分成4组：Normal&#x2F;CA&#x2F;DSS&#x2F;DSS+CA</p>
<blockquote>
<ul>
<li>CA如何影响肠屏障完整性<blockquote>
<ul>
<li>TUNEL&#x2F;DAPI染色检查这几组小鼠的上皮细胞凋亡</li>
<li>qPCR检测ISC亚群相关转录因子的表达（如Lgr5、Dclk和Bmil1、Sox9和Lrig1）</li>
<li>透射电子显微镜观察上皮细胞形态学变化（可见上皮细胞排列、杯状细胞位置、潘氏细胞形态、粘液和嗜酸性胞质颗粒、线粒体形态、隐窝结构变化）；</li>
</ul>
</blockquote>
</li>
<li>CA对肠道ISC细胞功能的改变<blockquote>
<ul>
<li>从这四组小鼠分离肠隐窝体外培养产生的突起、出芽情况反应类器官形成能力【病理浓度CA显著减弱结肠炎小鼠隐窝类器官的出芽和传代能力】</li>
<li>流式细胞术检测各组小鼠肠道隐窝中Lgr5-EGFP+ 细胞比例【CA直接抑制Lgr5-EGFP+ 细胞增殖】</li>
<li>免疫荧光Edu和DAPImerge、Olfm4染色检查肠上皮干细胞增殖情况（补充CA的结肠炎小鼠隐窝中Edu+细胞和Edu+Olfm4+细胞数量减少）</li>
<li>CA和DMSO处理离体培养的Lgr5+ ISC，检测结肠形成效率以及Lgr5和Olfm4 mRNA表达【CA直接抑制Lgr5-EGFP+ 细胞增殖】</li>
</ul>
</blockquote>
</li>
</ul>
</blockquote>
</blockquote>
<blockquote>
<ul>
<li>AAV-GFP(对照组：DSS处理7d，water3d)和AAV-shCyp8b1-KD组</li>
<li>AAV-mCherry（对照组）和AAV-Cyp8b1-OE组<br>【CYP8B1敲低保留了DSS处理后有能力的Lgr5+ISC】<br>【AAV-shCyp8b1-KD结肠炎小鼠来源的隐窝具有更高的出芽和类器官形成能力】<br>【AAV-shCyp8b1-KD结肠炎小鼠的类器官则发生了次级类器官的重组】</li>
</ul>
</blockquote>
<blockquote>
<ul>
<li>WT和Cyp8b1-null</li>
<li>NC和Cyp8b1ΔHep【检测肠道ISC细胞标志基因表达】</li>
<li>IL10KO+vehicle和IL-10KO+CA</li>
<li>IL10KO+AAV-GFP(对照组)和IL-10KO +AAV-shCyp8b1-KD组【Il10-null模型中，CA补充和CYP8B1过表达均显著降低了Lgr5+ISC表达】</li>
</ul>
</blockquote>
<p><font face="Times New Roman">Results5</font>  <font face="楷体" color="black" size="4">CA通过抑制脂肪酸氧化损伤Lgr5 +肠道干细胞更新</font></p>
<blockquote>
<ul>
<li>从Lgr5-EGFP-IRES-creERT2小鼠中分离肠隐窝，分组CA处理组，DMSO对照组<blockquote>
<ul>
<li>qPCR检测与细胞周期、细胞分化和Wnt通路相关基因的mRNA的表达</li>
</ul>
</blockquote>
</li>
</ul>
</blockquote>
</article><div class="post-copyright"><div class="post-copyright__author"><span class="post-copyright-meta"><i class="fas fa-circle-user fa-fw"></i>文章作者: </span><span class="post-copyright-info"><a href="http://example.com">莫为轻阴便拟归</a></span></div><div class="post-copyright__type"><span class="post-copyright-meta"><i class="fas fa-square-arrow-up-right fa-fw"></i>文章链接: </span><span class="post-copyright-info"><a href="http://example.com/2024/01/31/%E7%AC%AC%E4%B8%80%E7%AF%87%E5%8D%9A%E5%AE%A2/">http://example.com/2024/01/31/%E7%AC%AC%E4%B8%80%E7%AF%87%E5%8D%9A%E5%AE%A2/</a></span></div><div class="post-copyright__notice"><span class="post-copyright-meta"><i class="fas fa-circle-exclamation fa-fw"></i>版权声明: </span><span class="post-copyright-info">本博客所有文章除特别声明外，均采用 <a href="https://creativecommons.org/licenses/by-nc-sa/4.0/" target="_blank">CC BY-NC-SA 4.0</a> 许可协议。转载请注明来自 <a href="http://example.com" target="_blank">莫为轻阴便拟归</a>！</span></div></div><div class="tag_share"><div class="post-meta__tag-list"></div><div class="post_share"><div class="social-share" data-image="https://i.loli.net/2021/02/24/5O1day2nriDzjSu.png" data-sites="facebook,twitter,wechat,weibo,qq"></div><link rel="stylesheet" href="https://cdn.jsdelivr.net/npm/butterfly-extsrc@1.1.3/sharejs/dist/css/share.min.css" media="print" onload="this.media='all'"><script src="https://cdn.jsdelivr.net/npm/butterfly-extsrc@1.1.3/sharejs/dist/js/social-share.min.js" defer></script></div></div><div class="post-reward"><div class="reward-button"><i class="fas fa-qrcode"></i>赞助</div><div class="reward-main"><ul class="reward-all"><li class="reward-item"><a href="/img/wechat.jpg" target="_blank"><img class="post-qr-code-img" src="/img/wechat.jpg" alt="wechat"/></a><div class="post-qr-code-desc">wechat</div></li></ul></div></div><nav class="pagination-post" id="pagination"><div class="next-post pull-full"><a href="/2024/01/29/hello-world/" title="Hello World"><div class="cover" style="background: var(--default-bg-color)"></div><div class="pagination-info"><div class="label">下一篇</div><div class="next_info">Hello World</div></div></a></div></nav></div><div class="aside-content" id="aside-content"><div class="card-widget card-info"><div class="is-center"><div class="avatar-img"><img src="https://i.loli.net/2021/02/24/5O1day2nriDzjSu.png" onerror="this.onerror=null;this.src='/img/friend_404.gif'" alt="avatar"/></div><div class="author-info__name">Mingyanlee</div><div class="author-info__description"></div></div><div class="card-info-data site-data is-center"><a href="/archives/"><div class="headline">文章</div><div class="length-num">2</div></a><a href="/tags/"><div class="headline">标签</div><div class="length-num">0</div></a><a href="/categories/"><div class="headline">分类</div><div class="length-num">0</div></a></div><a id="card-info-btn" target="_blank" rel="noopener" href="https://github.com/xxxxxx"><i class="fab fa-github"></i><span>Follow Me</span></a></div><div class="card-widget card-announcement"><div class="item-headline"><i class="fas fa-bullhorn fa-shake"></i><span>公告</span></div><div class="announcement_content">This is my Blog</div></div><div class="sticky_layout"><div class="card-widget" id="card-toc"><div class="item-headline"><i class="fas fa-stream"></i><span>目录</span><span class="toc-percentage"></span></div><div class="toc-content"><ol class="toc"><li class="toc-item toc-level-2"><a class="toc-link" href="#%E5%BB%BA%E7%AB%8B%E5%8D%9A%E5%AE%A2%E4%B9%8B%E5%90%8E%E6%80%8E%E4%B9%88%E5%81%9A"><span class="toc-number">1.</span> <span class="toc-text">建立博客之后怎么做</span></a></li><li class="toc-item toc-level-2"><a class="toc-link" href="#%E6%96%87%E7%8C%AE%E5%AD%A6%E4%B9%A0"><span class="toc-number">2.</span> <span class="toc-text">文献学习</span></a></li></ol></div></div><div class="card-widget card-recent-post"><div class="item-headline"><i class="fas fa-history"></i><span>最新文章</span></div><div class="aside-list"><div class="aside-list-item no-cover"><div class="content"><a class="title" href="/2024/01/31/%E7%AC%AC%E4%B8%80%E7%AF%87%E5%8D%9A%E5%AE%A2/" title="第一篇博客">第一篇博客</a><time datetime="2024-01-31T06:42:33.000Z" title="发表于 2024-01-31 14:42:33">2024-01-31</time></div></div><div class="aside-list-item no-cover"><div class="content"><a class="title" href="/2024/01/29/hello-world/" title="Hello World">Hello World</a><time datetime="2024-01-29T12:28:34.172Z" title="发表于 2024-01-29 20:28:34">2024-01-29</time></div></div></div></div></div></div></main><footer id="footer"><div id="footer-wrap"><div class="copyright">&copy;2020 - 2024 By Mingyanlee</div><div class="framework-info"><span>框架 </span><a target="_blank" rel="noopener" href="https://hexo.io">Hexo</a><span class="footer-separator">|</span><span>主题 </span><a target="_blank" rel="noopener" href="https://github.com/jerryc127/hexo-theme-butterfly">Butterfly</a></div></div></footer></div><div id="rightside"><div id="rightside-config-hide"><button id="readmode" type="button" title="阅读模式"><i class="fas fa-book-open"></i></button><button id="translateLink" type="button" title="简繁转换">简</button><button id="darkmode" type="button" title="浅色和深色模式转换"><i class="fas fa-adjust"></i></button><button id="hide-aside-btn" type="button" title="单栏和双栏切换"><i class="fas fa-arrows-alt-h"></i></button></div><div id="rightside-config-show"><button id="rightside-config" type="button" title="设置"><i class="fas fa-cog fa-spin"></i></button><button class="close" id="mobile-toc-button" type="button" title="目录"><i class="fas fa-list-ul"></i></button><button id="go-up" type="button" title="回到顶部"><span class="scroll-percent"></span><i class="fas fa-arrow-up"></i></button></div></div><div><script src="/js/utils.js?v=4.12.0"></script><script src="/js/main.js?v=4.12.0"></script><script src="/js/tw_cn.js?v=4.12.0"></script><script src="https://cdn.jsdelivr.net/npm/@fancyapps/ui@5.0.32/dist/fancybox/fancybox.umd.min.js"></script><div class="js-pjax"></div><script async data-pjax src="//busuanzi.ibruce.info/busuanzi/2.3/busuanzi.pure.mini.js"></script></div></body></html>